Course in Bern, Switzerland: Long-read Sequence Analysis
This course is addressed to anyone working with or interested in working with long-read data.
Application deadline: 19 February 2025
Date: 27 - 28 February 2025
Description
For the last few years, research in DNA and RNA has been dominated by short-read sequencing. Although this has revolutionized the way we answer biological questions, it has its limitations. One important limitation is read length. The invention of high-throughput long-read sequencing has enabled us to solve questions on genome assembly, haplotyping, structural variation and alternative splicing. In this course, the participant will get acquainted with the major long-read sequencing technologies (PacBio SMRT and Oxford Nanopore Technology), and get experience with hands-on work involving long-read data. The training materials for this course are in its dedicated GitHub page. The course includes a guest lecture from Dr. Alban Ramette and Dr. Pamela Nicholson.
General info
- Time: 27 - 28 February 2025
- Registration deadline: 19 February 2025
- Location: Bern, Switzerland
- Fees:
- Academic: 200 CHF
- For-profit: 1000 CHF
Course materials are freely available: https://sib-swiss.github.io/NGS-longreads-training/latest/
Prerequisites
- You should meet the learning outcomes of: First Steps with UNIX in Life Sciences or the e-learning course UNIX Fundamentals.
- In case of doubt, evaluate your Unix skills with this quiz.
- You are required to bring your own laptop. Software installation instructions will be communicated before the course.
Learning outcomes
- Understand the basics behind PacBio SMRT sequencing and Oxford Nanopore Technology sequencing
- Use the command line to perform quality control and read alignment of long-read sequencing data
- Be able to do differential isoform expression analysis or a repeat expansion analysis based on long-read sequencing data