Course in Switzerland: Introduction to bulk RNA-Seq: From Quality Control to Pathway Analysis
This course is addressed to life scientists or bioinformaticians familiar with “Next Generation Sequencing” who wish to acquire the necessary skills to analyse RNA-seq gene expression data. Course material is available for free.
Application deadline: 05 September 2024
Date: 19 - 20 September 2024
General information
- Location: Zürich, Switzerland
- Application deadline: 05 September 2024
- Date: 19 - 20 September 2024
- Level: Beginner
- Fees:
- Academic: 200 CHF
- For-profit: 1000 CHF
Description
This two-day course will present the theory and bioinformatics tools required to analyze RNA-seq gene expression data. The course will present the theory and bioinformatics tools required to analyse RNA-seq gene expression data, from the raw data until the biological interpretation. The following topics will be discussed:
- Quality control
- Mapping to genomes & transcriptomes
- Read counting, gene differential expression analysis
- GO enrichment and pathway analysis
Prerequisites
- Participants should already have a basic knowledge in Next Generation Sequencing (NGS) techniques.
- Basic knowledge of the R statistical software and of the UNIX fundamentals.
- You are required to have your own Wi-Fi enabled laptop.
- You should also have access to a terminal on your computer.
Learning outcomes
At the end of the course, the participants are expected to:
- Describe advantages and pitfalls of RNA sequencing
- Design their own experiment
- Perform the downstream analysis using command line software (QC, mapping, counting, differential expression analysis, pathway analysis, etc)
- Critically assess the quality of their results at each step of the downstream analysis